ISO 8128-2:1993 (R2022)
Active , Reaffirmed Standard
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ISO 8128-2:1993 (R2022)

Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content
Part 2: Method using thin-layer chromatography

Summary

The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.

Notes

90.93 : Norme internationale confirmée

Technical characteristics

Publisher International Organization for Standardization (ISO)
Publication Date 06/24/1993
Confirmation Date 01/20/2022
Edition 1
Page Count 5
EAN ---
ISBN ---
Weight (in grams) ---
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