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NBN ISO 19056-3:2022

Microscopes — Definition and measurement of illumination properties — Part 3: Incident light fluorescence microscopy with incoherent light sources

Summary

This document specifies procedures for the measurement of illumination brightness, temporal stability
and uniformity for incident light fluorescence microscopy. The measurement for uniformity is defined
in image planes or intermediate image planes only, when these planes are suitable for detection by
electronic imaging devices.
This document defines how illumination brightness, temporal stability and uniformity are measured,
and how this information is provided to the user.
NOTE The scope is intentionally limited to electronic imaging devices and (intermediate) image planes.
The visual observation by means of eyepieces would require a different measurement procedure and hence
result in ambiguities in the description of measurement procedures. Nevertheless, this document will give
useful estimates for the uniformity with visual observation as in this case an eyepiece is used to observe an
intermediate image plane (which is under the scope of this document).

Technical characteristics

Publisher Bureau de Normalisation Belge (NBN)
Publication Date 06/13/2022
Page Count 18
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ISBN ---
Weight (in grams) ---
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