NBN ISO 20397-3:2025

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:

a)       sampling strategy and process, including type, storage, transportation, extraction, quality;

b)       nucleic acid library preparation

c)        design and review process including sequencing strategy and assessment;

d)       database construction;

e)       bioinformatics analysis and report

f)         validation and verification for bioinformatics pipeline, and database

This document applies to laboratories and research organizations.